Fig 1: CD133 is a cancer stem cell marker in nonsmall cell lung cancer cells. (A) H460 NSCLC cells were grown as spheroids in stem cell media and flow sorted for very high (red) or very low (blue) CD-133 expression with the indicated gating strategy. The CD133 positive and CD133 negative cells were plated at limiting dilutions (0→128 cells /well) and assessed for spheroid formation (yes/no). (B) The CD133 positive and CD133 negative cells were implanted into mice in limiting dilutions and assessed for tumor formation (yes/no). (C) Tumor growth curves of mice injected with indicated number of flow-sorted cells. * indicates P < 0.05.